Western Blot

It is important to understand the advantages and disadvantages of different ways of detecting a protein of interest on a western blot membrane. Here, we consider the three main types of protein detection for western blot; radioactive, enzymatic, and fluorescent.

Download this guide to learn the importance of context in optimization, why controls can make or break an assay and how sample status may be confirmed in an antibody-independent manner.

This article outlines key workflow steps to help researchers perform better western blots. By executing best practices in these steps – sample preparation, gel electrophoresis, gel transfer, protein detection, and image analysis – researchers can expect good western blot data.

Download this guide to explore how you can achieve high-quality western blots by improving protein isolation and sample preparation, optimizing your antibodies and using controls effectively.