Tissue Dissociation for Single-Cell Sequencing

With recent technological advancements, single-cell sequencing can now allow tens of thousands of individual cells from a single tissue sample to be analyzed, giving researchers an unprecedented opportunity to understand individual cell populations and their behavior in diseased tissue. 

To retain the value of this process, tissue samples must be processed and the single cells isolated quickly to protect cell viability and minimize the changes within the cellular transcriptome. That said, the disaggregation process can still result in sample loss.  

Download this collection of case studies to learn how scientists used a tissue disaggregator to:

• Produce high-quality single-cell suspensions from heart tissue
• Dissociate both fresh and snap-frozen tissue into single-nuclei suspensions
• Generate a higher yield of viable mouse liver cells in less time when compared to a dissociator