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mRNA Snippet

Optimizing mRNA Purification Conditions by Using a High-Throughput Screening Approach

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To support the development of mRNA-based therapies scientists require platforms that will help to address the selectivity and capacity requirements for the large-scale manufacturing of mRNA used in vaccine and gene therapy applications.

Typically, mRNA binds to the Oligo dT(25) affinity resin using high ionic strength conditions and neutral pH and is eluted from the column using low ionic strength solutions like water. Although water works well for most mRNA constructs, a need to identify alternative elution buffers which can be used to optimize mRNA recovery, exists.

Watch this 30-minute webinar to discover a resin that enables a simplified mRNA purification workflow and maximizes the removal of the DNA template and other IVT components. 

Watch this webinar to:

  • Learn how to prepare and implement a high-throughput screening approach to identify alternative elution buffer conditions
  • Understand how the ionic strength of the binding conditions can impact the recovery
  • Learn how to apply the optimized purification method towards various lengths of mRNA constructs


For Research Use Only. Not for use in diagnostic procedures.

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