Novel Aggregation-Specific Fluorogen Monitors Prefibrillar Protein Aggregation by Fluorescence Polarization

Highly ordered protein aggregates, termed amyloid fibrils, are associated with a broad range of diseases, many of which are neurodegenerative for example Alzheimer’s and Parkinson’s. The transition from soluble, functional protein to insoluble amyloid fibril occurs via a complex process involving the initial generation of highly dynamic early stage aggregates or prefibrillar species. Prefibrillar species are proposed to play a key role in the cytotoxicity of amyloid fibrils. Therefore, novel probes that have broad applicability in the detection of prefibrillar species of amyloidogenic proteins are actively being sought.

Download this app note to discover a novel broad-spectrum fluorescent probe that:

• Can be used to monitor prefibrillar species of amyloidogenic proteins
• Has superior characteristics to Thioflavin T