ioGABAergic Neurons – iPSC-Derived Inhibitory Neurons With >95% Purity

ioGABAergic Neurons are human induced pluripotent stem cell (iPSC)-derived inhibitory GABAergic neurons, precision reprogrammed using opti-ox™technology, that express classical marker genes including GAD1, GAD2, VGAT, DLX1 and DLX2. These cells provide an easy to use, high-quality, co-culture compatible human model for the study of neurological circuitry, neurodevelopmental (autism, epilepsy) and neurodegenerative (Alzheimer’s) disease.

Using a simple 2-step protocol, consistently go from cryopreserved cells to mature, functional, experiment-ready GABAergic neurons with over 95% purity in just 12 days, as confirmed by single cell RNA sequencing. The high purity of ioGABAergic Neurons solves the problem of heterogeneous neuronal populations, obtained with traditional directed differentiation protocols, where excitatory neurons often obscure inhibitory signals in cell-based assays.

Obtain reproducible results from your assays, regardless of the lot, assay, user, or lab, and save valuable time on batch validation. No prior experience of culturing iPSC-derived cells is required as every vial of ioGABAergic Neurons will consistently generate a highly defined cell population, as confirmed by bulk RNA sequencing.

Calcium imaging has been used to demonstrate that ioGABAergic Neurons display spontaneous activity.  In the human brain, the functions of GABAergic neurons are intimately connected with glutamatergic neurons and astrocytes, making co- or tri-culturing these cell types desirable in vitro. ioGABAergic Neurons are suitable for co- and tri-culture studies with astrocytes and glutamatergic neurons, to more closely mirror and study in vivo brain function.

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