Latest App Notes & Case Studies

Oxidative stress has been demonstrated to be involved in many diseases including atherochlerosis, Parkinson disease and cancer. Oxidative stress is caused by an imbalance between the production of reactive oxygen and a biological system’s ability to detoxify the reactive intermediates, or easily repair the resulting damages.

Melamine is an industrial chemical most commonly used as a fire retardant in dry powder extinguishers due to its release of nitrogen gas when burned or charred. Melamine has been used illegally by adding it to food products in order to increase the apparent protein content.

The Thermo Scientific KingFisher Flex magnetic particle processor is specifically designed to automate the time-consuming sample preparation of proteins, nucleic acids and cells in 96-well plate formats.

The purification of DNA and RNA from clinical samples requires rapid and reliable processing of large quantities of samples. Automated purification of nucleic acids, proteins and cells with Thermo Scientific KingFisher Flex is a reproducible and high quality method, which produces excellent material for further analyses.

This application note describes how to perform photometric DNA quantitation and qualification measurements with the Thermo Scientific Multiskan GO microplate and cuvette spectrophotometer in various cuvette formats.

One of the most common luminometric assays is Dual Luciferase® Reporter Assay from Promega corp. This application note shows how this assay is performed with Varioskan Flash multitechnology microplate reader. The note includes the performance of the normal DLR assay and results from some tests showing important aspects of the basic behaviour and performance of the DLR™ chemistry. This paper describes the overall efficiency of the Varioskan Flash in the measurement of DLR assay.

Potassium bromate is recognised as one of the best dough conditioners in the bakery industry. Scientific evidence surfaced in 1990, implicating bromate as a possible carcinogen. In response the United Nations Joint Food and Agricultural Organisation and the WHO reduced bromate residuals to less than 5 parts per billion (ppb) in finished products.

Tandem chromatography is emerging as a powerful technique to double the output of HPLC equipment by simultaneously carrying out two different analyses on the same sample. The principle is straightforward: the sample is split in two at the injection port, and each aliquot is eluted on a different column and analysed by a different detector.

Sudan I is the common name given to 1-phenylazo-2-naphthol, a fat-soluble dye used industrially to colour oils, waxes, greases, varnishes etc. It has been recognised by health authorities as being potentially carcinogenic, and is banned as a food additive in the UK and across the European Union.

Inorganic cations are significant indicators of the quality of drinking waters and their suitability for human consumption. They have important physiological effects and they alter the palatability of water.