Latest App Notes & Case Studies

Energy metabolism plays a central role in a wide variety of cellular and physiological processes. Therefore, a deep understanding of how disruption to energy metabolism impacts cells can help in therapeutic drug discovery and development.

Download this app note to discover a method of recombinant protein expression that enables correct protein folding and post translational modification, is suitable for high-density cell culture, and can produce both secreted and intracellular proteins of various species.

There is currently no effective cure or treatment to slow down or stop the progression of Huntington's disease (HD). One reason for this is the lack of accurate and easy-to-use HD models.

Proteolysis targeting chimera (PROTAC) degraders are used to treat drug-resistant targets via targeted protein degradation.

Rare cells play pivotal roles in disease initiation, maintenance and progression. Yet, identifying these cells remains a difficult task for low-plex technologies, as it is not always clear a priori which biomarkers distinguish rare phenotypes from the remaining population.

In drug screening, conventional high-throughput applications evaluate only a few measurements and have limited breadth to detect diverse phenotypic changes caused by compound exposure.

Pyruvate kinase is an enzyme involved in energy-supplying glycolysis and ATP synthesis, thus is pivotal for the eucaryotic energy metabolism. In addition, overexpression of pyruvate kinase is believed to play a role in cancer.

One of the major challenges faced by most toxicology laboratories is analyzing hundreds of drugs of abuse in biological samples. This is further complicated by the constantly increasing emergence in the volume of designer and illicit drugs.

RTP004 is a proprietary peptide used by Revance Therapeutics as a novel excipient in DaxibotulinumtoxinA for injection. Due to its high molecular weight, high isoelectric point and strong non-specific binding property, the sample preparation and LC-MS method development for this peptide is very challenging.

In early-stage pharmaceutical research, knowledge of a compound’s metabolism can assist in the identification of structural liabilities that can contribute to non-optimal pharmacokinetics, potentially toxic breakdown products or the potential for drug-drug interactions.