Latest Posters
Poster
MicroRNA Expression Signature in Human Glioblastoma Multiforme Brain Tumor
Expression of 180 human miRNAs was examined using recently developed stem loop primers for reverse transcription (RT) followed by real-time PCR. MicroRNAs can be quantified from as few as single cells or as little as 25 pg total RNA.
Poster
rptDB; a prototype Affymetrix .rpt QC tool
rptDB is a prototype database for the analysis of Affymetrix expression report (.rpt) files. Report files are widely used to QC raw data after hyb, scan and .cel file generation. Only %Present calls, Scale Factors and 3':5' ratios of housekeeping genes are generally used, but there are many other metrics that may also be of importance for QC analysis.
Poster
Development of low Cost High Throughput miRNA Screening
Micro-RNAs (miRNAs) form a class of 17-24 base singles trandnon-coding RNAs which regulate gene expression post-transcriptionally in eukaryotes and viruses. We have developed a low cost high throughput method to quantify the expression of these miRNAs via classictotal RNA extraction without interference from genomic DNA.
Poster
A Mild Protocol for Parallel Solution Phase Synthesis of Cyclic Imides
The combination of using high temperatures and coupling reagents allows production of cyclic imides rapidly and in higher purity than heat or coupling agents alone.
Poster
Microwave-Accelerated Fischer Glycosidation
Fischer glycosidation appeared an ideal candidate for the application of microwave irradiation and herein we report a systematic analysis of the effect of microwave heating in the Fischer glycosidation reaction.
Poster
Chip’N’Fish : Novel Chemical Proteomics Platform for Rapid Identification of Small Molecule Drug Candidates Against Disease-Specific Targets
Over-expression of genes and their protein products is often the best starting point for dissecting the pathways that are responsible for the development of specific diseases.The Chip'N'Fish approach allows identifying over-expressed proteins directly from cell extracts of disease origin using healthy counterparts as controls.
Poster
High-Throughput Automation of a Dual Reporter Assay in Low Volume 384 & 1536 Well Plate Formats
The drive towards miniaturization within the pharmaceutical and biotechnology fields has created a need for liquid handling technologies that accurately deliver low volume reagents to high-density plates. This has also created a need for simple, fully scaleable assays in low volumes. Here we demonstrate the successful combination of both through the use of the Equator™ NS 808 - Eight Tip Pipetting System and the dual color Chroma-Luc™ technology.
Poster
Miniaturisation of Liquid Handling Procedures for High Throughput Sequencing
Deerac Fluidics Equator™ products are designed to fit a wide range of applications requiring low volume liquid handling. The products have recently been installed as part of the high throughput sequencing production process at the Broad Institute in Boston, MA, USA. This poster will describe the current process employed by Broad Institute, highlighting where the Equator™ has played a vital role in performance enhancement and cost reduction, enabling the Broad Institute to retain its position as
Poster
Software for Automated Differential Expression Using 2D and 3D Representations of LC-MS Data Interactive Confirmation of Results
DeCyder™ MS is a novel software program for fully automated differential expression analysis based on LC-MS/MS data including detection, quantitation, sample to sample comparison and statistical data evaluation. Results can be interactively confirmed against original raw data through 2D and 3D data visualizations.
Poster
The Use of Software Visualization Tools for the Identification of PTM Patterns and Artefacts in LC-MS Experiments
By representing LC-MS data as a 2D-image as demonstrated in the novel DeCyder™ MS software, it is possible to identify phenomena, which are very difficult to recognize using conventional LC-MS data representation.
Advertisement